
Fluorescent targets are used for the precise calibration and characterization of fluorescence microscopes. They enable reproducible x/y/z positioning as well as the calibration of sensitivity and resolution. Based on photoluminescent thin films in combination with high-precision apertures on glass, they generate defined and stable emission patterns. By carefully selecting the coatings, brightness, robustness, and durability can be optimized, resulting in long-lasting reference standards for applications in life sciences and diagnostics.
These solid-state solutions provide a robust and maintenance-free alternative to conventional liquid or particle-based calibration methods. Their high photostability and resistance to photobleaching ensure consistent performance even under intense illumination conditions. In addition, the structures can be precisely tailored in terms of geometry, emission wavelength, and intensity, allowing seamless integration into a wide range of microscopy systems. This makes them ideally suited not only for routine calibration tasks, but also for system validation, performance benchmarking, and advanced imaging applications.
